human normal pancreatic duct epithelial cell line hpde  (ATCC)

Journal: Molecules

Article Title: Gastrodin Induces Ferroptosis of Glioma Cells via Upregulation of Homeobox D10

doi: 10.3390/molecules28248062

Figure Lengend Snippet: HOXD10 as the key target of gastrodin. ( A ) Identified differentially expressed genes in glioma cells treated with DMSO and gastrodin. ( B ) Results of protein–protein interaction network analysis performed for differentially expressed genes. HOXD10 had strong relationship with other proteins encoded by differentially expressed genes. ( C , D ) Results of RT-qPCR and Western blotting performed to analyze the mRNA and protein levels of HOXD10 in glioma cells treated with different concentrations (0, 5, 10, and 20 μM) of gastrodin. ( E ) Results of Western blotting performed to analyze the protein levels of HOXD10 in glioma cells, HT22, C6 and PC12. ( F ) Results of qRT-PCR performed to analyze the mRNA levels of HOXD10 in glioma cells, LO2, HPDE, NHA, HK2, and HT22. ( G ) Binding mode of gastrodin with HOXD10 and 3D illustration of the details of the interaction. Green shows gastrodin; aquamarine shows the HOXD10 protein. * represents p < 0.05; ** represents p < 0.01; *** represents p < 0.001. n = 3. The control group was used for comparison. Data are shown as means ± SD.

Article Snippet: Immortalized mouse hippocampal cells (HT22), rat glioma cells (C6), human normal hepatocytes cells (LO2), human renal proximal tubular cells (HK2), human pancreatic duct epithelial cells (HPDE), normal human astrocytes (NHA), and human glioma cell lines (U251, T98, and LN229) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Quantitative RT-PCR, Western Blot, Binding Assay, Control, Comparison

Journal: Cell Death & Disease

Article Title: The MYEOV-MYC association promotes oncogenic miR-17/93-5p expression in pancreatic ductal adenocarcinoma

doi: 10.1038/s41419-021-04387-z

Figure Lengend Snippet: a , b Kaplan–Meier curves of overall survival ( a ) and disease-free survival ( b ) of patients with PDAC based on MYEOV expression in the GEPIA database using the log-rank test. The dotted lines indicate the 95% confidence intervals for survival percentage at each time point as obtained by survival analysis. c MYEOV expression levels in PDAC ( n = 178) and NCP tissues ( n = 171) based on the expression levels in GEPIA database. Gene expression profiles of MYEOV from all available samples classified as PDAC tissue ( n = 178) in TCGA and NCP tissue ( n = 171) in TCGA and GTEx were chosen to perform differential analysis in GEPIA. The data are presented as the means ± SEM. * P < 0.05 (one-way ANOVA). d ROC curves based on the gene expression profiles of MYEOV in PDAC and normal tissue samples. The AUC value with a 95% CI is indicated. e Representative images of the IHC staining analyses of 19 FFPE PDAC and NCP tissues using an anti-MYEOV antibody (400× magnification). f Comparison of MYEOV expression levels in PDAC cell lines and normal pancreatic epithelial cells by qPCR. All n ≥ 3; bar, SEM; *** P < 0.001; Student’s t -test. ANOVA, analysis of variance; PDAC, Pancreatic ductal adenocarcinoma; NCP, non-cancerous pancreas; TPM, transcripts per kilobase million; ROC, receiver operating characteristic.

Article Snippet: The human PDAC cell lines MIAPaCa-2, BxPC-3, PANC-1 and AsPC-1, as well as normal human pancreatic duct epithelial (HPDE) cells, were obtained from ATCC (Manassas, VA, USA) and cultured according to the manufacturer’s instructions.

Techniques: Expressing, Gene Expression, Immunohistochemistry, Comparison